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For renal impairment, although most GLP-1 agents have minimal renal clearance, impaired kidney function increases the risk of adverse events such as dehydration from vomiting or decreased drug clearance in polypharmacy

The vital dye 2-(4-(dimethylamino)styryl)- N -ethylpyridinium iodide (DASPEI) (Life Technologies, Grand Island, NY) is a marker of mitochondrial membrane potential and preferentially stains lateral line hair cells when added to the surrounding EM (Harris et al., 2000) per fish were assessed based on fluorescent intensity (Harris et al., 2009)